| A nested-PCR protocol for detection of the chicken anemia virus. S Simionatto, CA Lima-Rosa, LL Rubin, CW Canal Pesquisa Veterinaria Brasileira 25:22, ... www.cababstractsplus.org/ |
| ScientistSolutions provides a forum for discussing apoptosis, cytokine receptors, GPCR, RAS/RAF/ERK. STAT/JAK pathways, T-cell receptors, kinases and more. www.scientistsolutions.com/ |
| File Format: Microsoft Word - View as HTML PCR PROTOCOL. For a 25ul rxn:. Use 1ul of 60ng/ul or 100ng/ul DNA; Use 1ul of each primer at 3.2pmole/ul concentration or 1.25ul of each primer at 100ng/ul ... microgen.ouhsc.edu/manuals_protocols/pcr_protocol.doc - |
| DNA computing often requires oligonucleotides that do not produce erroneous cross hybridizations. By using in vitro evolution, huge libraries of non ... citeseer.ist.psu.edu/705429.html - 17k - |
| File Format: Microsoft Word - View as HTML QRT-PCR protocol. General notes:. Consistency is crucial to the accuracy of QRT-PCR, so the extra steps in this protocol are necessary. ... www.medmicro.wisc.edu/ |
| A Novel RT-PCR Protocol for the Detection of Natural Waterborne Human Reoviruses: Speakers from Round Table Group's Speakers' Bureau. www.roundtablegroup.com/ |
| File Format: PDF/Adobe Acrobat - View as HTML True lab QRT-PCR protocol 01/05. This protocol uses the Stratagene Brilliant QPCR kit (cat # 600548 or 600552) with SYBR green to estimate ... life.bio.sunysb.edu/ee/truelab/ |
| File Format: PDF/Adobe Acrobat Copyright 2006, Applied Biosystems All rights reserved. For Research Use Only. Not for use in diagnostic procedures. NOTICE TO PURCHASER: LIMITED LICENSE ... docs.appliedbiosystems.com/pebiodocs/04310251.pdf - |
| File Format: PDF/Adobe Acrobat - View as HTML Commentary. Optimization of PCR Protocol in Microsatellite. Analysis with Silver and SYBR. ®. Stains. MUHAMMAD H. RAHMAN. 1. , BARRY JAQUISH ... pubs.nrc-cnrc.gc.ca/ispmb/ispmb18/R00-053.pdf |
| Stay updated on the latest technologies and news with Biocompare's newsletters. (See samples here.) Life Science. Molecular Biology. Immunology ... www.biocompare.com/protocols/protocol/ |
| Universal diagnostic RT-PCR protocol for arboviruses. Kuno G. Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, ... www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed& |
| Protocol for PCR amplification of formalin-fixed paraffin embedded animal tissues using the Extract-N-Amp Tissue PCR kit. www.sigmaaldrich.com/.../DNA_and_RNA_Purification/ |
| File Format: Microsoft Word - View as HTML Protocol for Real-Time RT-PCR. This protocol describes the detailed experimental procedure for real-time RT-PCR using SYBR Green I as mentioned in Xiaowei ... pga.mgh.harvard.edu/primerbank/PCR_protocol.doc - |
| A PCR-based Protocol for In Vitro Selection of. Non-crosshybridizing Oligonucleotides. Russell Deaton. 1. , Junghuei Chen ... www.springerlink.com/index/7apx1t48lbqq9vr3.pdf - |
| File Format: PDF/Adobe Acrobat - View as HTML PCR Protocol Form. Please fill in names and concentrations of reagents. Also, fill in the amount. (in uL) used in the PCR reaction. Master Mix Reagents: ... www.mdanderson.org/pdf/nucacid_protocol.pdf |
| File Format: Unrecognized - View as HTML Colony PCR Protocol. 1. Pull out eight glycerol stock plates from the –80oC freezer and set on bench top to thaw. Be sure to remove the foil seal before ... microarrays.berkeley.edu/file_download.php?fid=13 |
| Protocols > PCR. Standard PCR reaction. Steps for Standard PCR Reaction. Design primers. In general, primers should have the following properties: ... www.addgene.org/pgvec1?f=v& |
| PCR Protocol (For one 96 well plate):. Materials Needed:. PCR mix; Thermocycler 96-well plate with reusable lid; Peltier PCR 9700 machine and program ... medicine.ucsd.edu/faculty/ |
| File Format: PDF/Adobe Acrobat - View as HTML Hyma and Whittam. December 2004. Multiplex PCR Protocol for selective amplification of a new pathogenic lineage of. Escherichia which includes Escherichia ... www.shigatox.net/stec-new/protocols/PCR_EB.pdf |
| Reagent / Constituent, Concentration, Volume (µL). buffer (Qiagen), 10X, 2.0. MgCl2 (Qiagen), 15 mM, 0.8. dNTPs (Qiagen), 2.5mM, 1.6 ... www.mmrrc.org/strains/Gensat/EGFP_PCR.html - 8k - |
Appl Environ Microbiol, July 1998, p. 2545-2553, Vol. 64, No. 7
0099-2240/98/$00.00+0
National Research Council1 and National Health and Ecological Effects Research Laboratory,2 U.S. Environmental Protection Agency, Western Ecology Division, Corvallis, Oregon 97333, and Institute for Bioscience, Bioinformatics, and Biotechnology, George Mason University, Manassas, Virginia 201103
Pseudomonas species are plant, animal, and human pathogens; exhibit plant pathogen-suppressing properties useful in biological control; or express metabolic versatilities valued in biotechnology and bioremediation. Specific detection of Pseudomonas species in the environment may help us gain a more complete understanding of the ecological significance of these microorganisms. The objective of this study was to develop a PCR protocol for selective detection of Pseudomonas (sensu stricto) in environmental samples. Extensive database searches identified a highly selective PCR primer pair for amplification of Pseudomonas 16S rRNA genes. A protocol that included PCR amplification and restriction analysis, a general cloning and sequencing strategy, and phylogenetic analyses was developed. The PCR protocol was validated by testing 50 target and 14 nontarget pure cultures, which confirmed the selectivity to 100%. Further validation used amplification of target sequences from purified bulk soil DNA followed by cloning of PCR products. Restriction analysis with HaeIII revealed eight different fragmentation patterns among 36 clones. Sequencing and phylogenetic analysis of 8 representative clones indicated that 91.7% of the products were derived from target organisms of the PCR protocol. Three patterns, representing only 8.3% of the 36 clones, were derived from non-Pseudomonas or chimeric PCR artifacts. Three patterns, representing 61.1% of the clones, clustered with sequences of confirmed Pseudomonas species, whereas two patterns, representing 30.6% of the clones, formed a novel phylogenetic cluster closely associated with Pseudomonas species. The results indicated that the Pseudomonas-selective PCR primers were highly specific and may represent a powerful tool for Pseudomonas population structure analyses and taxonomic confirmations.
Full article here
| GM4JJJ. Amateur Radio. Home /; Icom PCR-1000 /. Home · About · Astronomy · Radio Software · Opthalmology Software · Icom PCR-1000 · Moonbounce · Microwave ... www.gm4jjj.co.uk/PCR1000.html - 45k - |
| Access the most recent version at doi:. 1992 1: 195-198. PCR Methods Appl. K J Mutchler, S W Klemish and A F Russo. genes from a cDNA library. ... www.genome.org/cgi/reprint/1/3/195.pdf?ck=nck |
| Access the most recent version at doi:. 1993 3: 211-212. PCR Methods Appl. F Capon, S L Cicero, G Novelli and B Dallapiccola. biopsies. ... www.genome.org/cgi/reprint/3/3/211.pdf |
| File Format: PDF/Adobe Acrobat ABgene 2003: 2-step-QRT-PCR.doc. 2-Step QRT-PCR Protocol. Method for use with:. •. Reverse-iT™ RTase Blend (AB-0914) ... www.abgene.com/downloads/Guide_2-step-QRT-PCR.pdf - |
| Protein Engineering vol.10 no.9 pp.1099–1100, 1997. PROTOCOL. A three-step PCR protocol for construction of chimeric proteins. Rita Grandori ... peds.oxfordjournals.org/cgi/reprint/10/9/1099.pdf |
| Karger is a medical publisher, scientific publisher and biomedical publisher of print and online journals and books. content.karger.com/ProdukteDB/produkte.asp?Doi=23379 |
| Leukemia is one of the leading journals in hematology and oncology. It is published monthly and covers all aspects of the research and treatment of leukemia ... www.nature.com/leu/journal/v20/n5/full/2404174a.html |
| PCR protocol for amplification of MLST genes. 1) For each 1005l PCR, use the following reaction components (multiply these volumes by the number of PCR ... pubmlst.org/neisseria/mlst-info/nmeningitidis/pcr.shtml - 10k - |
| Check our FAQs; Or contact us to report problems with:; Subscription access · Article delivery · Registration · Library administrator tasks · Other problems ... www.ingentaconnect.com/content/ |
| Quick Search:, within. All Full-text Sources. Quick Search searches abstracts, titles, keywords, and authors. Click here for more ... linkinghub.elsevier.com/retrieve/pii/S0015028205025057 |
| Figures and Tables. PDF (619K) · Contents · Archive · Journal Homepage. Related material:. PubMed record, PubMed related arts, PubMed LinkOut, Nucleotide ... www.pubmedcentral.nih.gov/ |
| Introduction · Research Pages · People involved Photo galleries Publications Other sample sites Education · Links to other micobial observatories ... www.hawaii.edu/microbiology/MO/longpcr.htm - 22k - |
| This Article. Right arrow, Full Text Freely available. Right arrow, Print PDF (78K) Freely available. Right arrow, Supplementary Data. Right arrow ... nar.oxfordjournals.org/cgi/content/abstract/27/6/1566 - |
| [edit] Anneal Extend PCR. This is a protocol for linking any 2 pieces of DNA together using homologous primers. The original protocol can be found here[1]. ... openwetware.org/wiki/Anneal_Extend_ |
| Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange Site-Directed Mutagenesis. Wang W, Malcolm BA. ... www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed& |
| (per Adam 08/12/04). TAIL is a series of reactions that are intended to map where a T-DNA (transfer DNA) has inserted within the genome. ... preuss.bsd.uchicago.edu/protocols/tail.html - 18k - |
| (per Adam 08/12/04). PCR (polymerase chain reaction) is a sensitive procedure that amplifies specific regions of DNA. A forward and reverse primer flank a ... preuss.bsd.uchicago.edu/protocols/PCR.html - 4k - |
| File Format: PDF/Adobe Acrobat - View as HTML DAU/GRC/NIA/NIH. 9/1/02. PCR PROTOCOL FOR cDNA ARRAYS ON MEMBRANES. Purpose: to amplify insert DNA from purified plasmid DNA derived from bacterial ... www.daf.jhmi.edu/microarray/protocols/protocol6.pdf |
| File Format: PDF/Adobe Acrobat - View as HTML RTPCR.doc. The Wellcome Trust Sanger Institute. RT-PCR protocol. RNA preparation. 1. Grow cells to confluence in a single well of a 6-well plate. ... www.sanger.ac.uk/PostGenomics/ |
| Optimized Welsh DDRT-PCR Protocol ... wheat.pw.usda.gov/~lazo/methods/lazo/ddrtpcr2.html - 17k - |
| Protocol for competitive RT-PCR. For quantifying mRNA, we use a competitive RT-PCR protocol with internal standard RNAs. These are added in a defined ... www.uni-ulm.de/~dkaufman/rt-pcr.html - 8k - |
| File Format: PDF/Adobe Acrobat - View as HTML THE INSTITUTE FOR GENOMIC RESEARCH. Standard Operating Procedure. Proprietory Information. TITLE: Two Step. REAL TIME RT-PCR PROTOCOL. PAGE: 1 of 5 ... pga.tigr.org/sop/RT-PCR.pdf |
| File Format: PDF/Adobe Acrobat - View as HTML Basic PCR Protocol. CGLab, 7/2002. 1). Wipe down the bench area with bleach and a new paper towel. 2). Take the PCR components out of the freezer to thaw ... www.sfsu.edu/~biology/ |
Don Liu, Cecilia Schmidt, Tim Billings, and Muriel T. Davisson, The Jackson Laboratory
Genotyping Ts65Dn mice is based on doing simultaneous quantitative PCR amplification of a gene or genes in the Ts65Dn chromosome and a control gene on another chromosome (in this case Apob) and comparing the average change (delta) in threshold cycle (CT) between the Ts65Dn genes and the control gene. Doing a multiplexed reaction with an internal control avoids the need for determining DNA concentration and permits a relatively large range of variation in concentration of template DNA.
Although trisomic females vary in transmission of the trisomy the overall incidence of trisomy among progeny is only 19-25%. To streamline the typing process further the animals may be visually phenotyped before they are genotyped. The phenotype is variable and subtle but to the trained eye the trisomic mice can be identified. Ts65Dn mice are usually smaller than their control littermates at weaning. When one lifts the cage cover slowly, the trisomic mice often raise their heads and bring their ears to the side. When picked up by the tail some trisomic mice make a high-pitched continuous squeak. Ts65Dn mice often display stereotypic behavior, repeatedly jumping up and down by the side of the cage. When a pencil is placed vertically in front of the mouse, controls will run around it but Ts65Dn mice sit back on their haunches and "chatter". Phenotyping the mice is not 100% accurate nor should it be relied upon to classify mice, but it does help eliminate control animals that are not needed and reduce the number of mice that must be genotyped. The combination of visual phenotyping followed by qPCR genotyping should make identifying Ts65Dn mice easier than chromosomal typing for most researchers.
Full protocol here
| Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA. www.biology.lsa.umich.edu/research/ |
| Protocol for PCR with Taq DNA Polymerase. Avoiding Contamination. PCR allows the production of more than 10 million copies of a target DNA sequence from ... www.fermentas.com/techinfo/pcr/dnaamplprotocol.htm - 22k - |
| File Format: PDF/Adobe Acrobat - View as HTML I. Protocol for PCR with Hot Start Taq DNA Polymerase. How to Avoid Contamination. During PCR, usually more than 10 million copies of a template DNA can be ... www.fermentas.com/profiles/modifyingenzymes/ |
| Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA. arep.med.harvard.edu/labgc/estep/longPCR_protocol.html Cached |
| The Jackson Laboratory Cytogenetic Models Resource maintains and distributes chromosome aberration stocks that provide primarily mouse models for Down ... www.jax.org/cyto/quanpcr.html - 32k - |
