Design of a degenerate PCR primer pair to amplify signature

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(1) Design of a degenerate PCR primer pair to amplify signature ...

File Format: Microsoft Word - View as HTML (1) Design of a degenerate PCR primer pair to amplify signature sequences for all vertebrate sarcomeric myosin heavy chain genes : Recognition of the MYH 16 ... www.nature.com/nature/journal/v428/n6981/extref/nature02358-s1.doc -

Use of Degenerate PCR

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Use of Degenerate PCR to Identify an Anti-apoptotic Gene of BHV-1 ...

We have undertaken the identification of a BHV-1 anti-apoptotic gene homologous. to c34.5 of HSV-1, using the degenerate PCR method. ... www.springerlink.com/index/X9012U4016U47J07.pdf -

Degenerate and Nested PCR

Degenerate and Nested PCR: a Highly Sensitive and Specific Method ...

In summary, we have established a highly sensitive and comprehensive degenerate PCR methodology for detection and genotyping of HPV from the skin and have ... jcm.asm.org/cgi/content/abstract/37/11/3545

Degenerate PCR and its application in gene cloning

[Degenerate PCR and its application in gene cloning]

Degenerate PCR is introduced in this paper, including what is degenerate PCR, how to design degenerate primers, how to optimize degenerate PCR parameters, ... www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=15639854&dopt=AbstractPlus -

Degenerate PCR

Degenerate PCR

The identification of novel members of gene families by PCR using degenerate primers is described and protocols given. Article by Michael Koelle 1996 on the ... www.dartmouth.edu/~ambros/protocols/other/koelle/degenerate_PCR.html - 17k - Cached -

Degenerate PCR

Degenerate PCR

An introduction with principle, applications and description of the technique. From the web site of Kebangsaan University, Malaysia. cgat.ukm.my/protease/degpcr.html - 11k - Cached -

Keunhwa's Inverse PCR Protocol

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Keunhwa's Inverse PCR Protocol

File Format: Microsoft Word - View as HTML Keunhwa's Inverse PCR Protocol. 1. Extract plant genomic DNA using the Qiagen DNeasy kit. Elute in 50㎕ EB buffer. 2. Digest 15㎕ DNA sample (~ 300ng) with ... bio.kaist.ac.kr/~pdbl/Inverse.doc -

Inverse PCR-Based RFLP Scanning

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Inverse PCR-Based RFLP Scanning Identifies Low-Level Mutation ...

We describe inverse PCR-based, amplified restriction fragment ... high-throughput inverse PCR in conjunction with automated denatur- ... cancerres.aacrjournals.org/cgi/reprint/64/7/2544.pdf -

Application of inverse PCR

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Application of inverse PCR to site-selected mutagenesis of Drosophila

Inverse PCR, by contrast, amplifies DNA on both. sides of the transposon (given restriction sites in suitable. proximity). Insertions are clearly followed ... nar.oxfordjournals.org/cgi/reprint/22/16/3429.pdf -

Inverse PCR and Sequencing Protocol

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Inverse PCR and Sequencing Protocol

File Format: PDF/Adobe Acrobat - View as HTML Inverse PCR and Sequencing Protocol on 5 Fly Preps. For recovery of sequences flanking XP elements. This protocol is an adaptation of ... flystocks.bio.indiana.edu/pdfs/Exel_links/5__fly_iPCR_XP_pub.pdf -

Inverse PCR Protocols - Biocompare

Inverse PCR Protocols - Biocompare

Browse Inverse PCR protocol categories listed below. ... Inverse PCR & Cycle Sequencing Of P Element Insertions For STS Generation Source: E. Jay Rehm, ... www.biocompare.com/protocols/category/3101/Inverse-PCR.html - 29k - Cached -

Inverse PCR primer design

Inverse PCR primer design

Inverse PCR is used to clone sequences flanking a known sequence. Flanking sequences are digested and ligated to make a circular DNA. ... changbioscience.com/primo/primoinv.html - 10k - Cached -

Inverse PCR & Cycle Sequencing

BDGP: Inverse PCR & Cycle Sequencing of P Element Insertions for ...

Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA. www.fruitfly.org/about/methods/inverse.pcr.html - 13k - Cached -

Inverse PCR

Inverse PCR For use with Snyder mTn-lacZ/LEU2 Based Mutagenesis

Protocol by M McMurray, Fred Hutchinson Cancer Research Center, Seattle, Wa. USA. www.fhcrc.org/labs/gottschling/misc/ipcr.html - 18k - Cached -

Inverse PCR protocol

Inverse PCR protocol

step by step protocol, from the web site of the Department of Biology, University of Michigan, USA. www.biology.lsa.umich.edu/research/labs/maddock/protocols/PCR/inverse_pcr_protocol.html - 4k - Cached

Yeast Single Colony PCR

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Yeast Single Colony PCR

File Format: Microsoft Word - View as HTML Use 2µl DNA per 20µl PCR reactions. Store the rest at –20°C. If your genomic preps aren't working – try a range of DNA amounts in your PCR reaction. www.bio.brandeis.edu/goodelab/protocols/Fast%20and%20Efficient%20Yeast%20Genomic%20DNA%20Preps.doc -

Colony PCR Method

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Colony PCR:

File Format: Microsoft Word - View as HTML Colony PCR:. A quick diagnostic for inserts within a plate of colony. PCR reagents w/ NENBiolabs:. Thermo buffer 2.5ul 1ul. DNTP 2.5ul 1ul. Taq .25ul .1ul ... www.dartmouth.edu/~yehlab/protocols/Molecular%20biology/Colony%20PCR.doc -

Colony PCR Protocol

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Colony PCR Protocol

File Format: Microsoft Word - View as HTML Colony PCR Protocol. Overnight Culture 5.0µl. 10x PCR buffer 5.0µl. MgCl2 (25mM) 5.0µl. Forward primer (10µM) 0.2µl. Reverse primer (10µM) 0.2µl ... www.unc.edu/~fconlon/Protocols/Colony%20PCR%20Protocol.doc -

Colony PCR PPT

[PPT]

Colony PCR

File Format: Microsoft Powerpoint - View as HTML Colony PCR. CPSC265 Class 8. Cloning. Cloning is the way in which we can take a single molecule, and make lots of bacterial cells that contain an identical ... stan.cropsci.uiuc.edu/courses/cpsc265/class8.ppt -

Yeast Whole Colony PCR (APG modifications)

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Yeast Whole Colony PCR (APG modifications) 1.

File Format: PDF/Adobe Acrobat - View as HTML Yeast Whole Colony PCR (APG modifications). 1. Pick a small amount of cells with a pipet tip (fresh colonies seem to work better) ... gasch.genetics.wisc.edu/protocols/zymo_whole_yeast_PCR.pdf -

Plasmid Colony PCR Leslie Vosshall

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Plasmid Colony PCR Leslie Vosshall [7/31/2001] 1. Set up PCR ...

File Format: PDF/Adobe Acrobat - View as HTML For each colony, you will need 20 ul of PCR master mix. ... Transfer 2 ul of resuspended colony into PCR tube. Make sure colony is evenly. resuspended. ... vosshall.rockefeller.edu/protocols/PlasmidColony.pdf -

COLONY PCR PROTOCOL

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IV.G.19 COLONY PCR This procedure can be used to amplify DNA from ...

File Format: PDF/Adobe Acrobat - View as HTML 2. Dispense 15ml mix per well of a 96-well PCR plate. Keep on ice. 3. Twirl a sterile toothpick or pipet tip in an individual colony, make an "X" streak ... www.biochem.northwestern.edu/.../research/Protocols/IV.%20DNA/G.%20Amplification/4.%20Colony%20PCR.pdf -

E. coli Colony PCR

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E. coli Colony PCR

File Format: PDF/Adobe Acrobat - View as HTML E. coli Colony PCR. 1. Pick colony with toothpick and swirl into 25 µl of TE (or dH. 2. O) in microfuge tube. 2. Heat in boiling water bath for 2 minutes ... plaza.ufl.edu/johnaris/Protocols/RNADNA/ColonyPCR.pdf -

Colony PCR Protocol

Colony PCR

Step by step protocol, from Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Israel. teachline.ls.huji.ac.il/72682/tutorials/baculovirus/protocols/Colony_PCR - 10k - Cached -

Colony PCR Protocol

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Colony PCR

File Format: PDF/Adobe Acrobat - View as HTML Colony PCR. This protocol is designed to quickly screen for plasmid ... To each cold PCR tube containing the PCR reaction, add a small amount of colony. To ... www.csun.edu/~mls42367/Protocols/Colony%20PCR.pdf -

Colony PCR

Colony PCR

Colony PCR is useful in determining whether or not a specific colony on a plate has a sequence you desire. Primers for the specific sequence should be used ... preuss.bsd.uchicago.edu/protocols/colony.html - 3k - Cached -

Colony PCR - OpenWetWare

Colony PCR - OpenWetWare

Colony PCR is can be used after a transformation to screen colonies for the desired plasmid. Primers are used which generate a PCR product of known size. ... openwetware.org/wiki/Colony_PCR - 12k - Cached -

Colony PCR

Colony PCR

Detailed protocol from the web site of the Department of Biology, University of Michigan, USA.
www.biology.lsa.umich.edu/research/labs/maddock/protocols/PCR/colony_pcr.html - 4k - Cached -

Yeast Colony PCR

Yeast Colony PCR

hsc banner. amberg lab. PROTOCOLS. Yeast Colony PCR ... Add 40 µl PCR reaction mix to the suspended colony:. 5 µl 5 mM gene specific primer 1 ...
www.upstate.edu/biochem/amberg/protocols/colony_pcr.html - 5k - Cached -

Colony PCR

Colony PCR

Learn about Colony PCR. Polymerase Chain Reaction on colonies of bacteria, E.coli, and Yeast.
www.pcrstation.com/colony-pcr/ - 19k - Cached -

Animations on PCR

Animations on PCR

Animation of RT-PCR. Animation of PCR. Animation of nucleic acid sequencing · Animation of PCR. Animation of PCR. Use the pop up menu below to access a ...
www.clinical-virology.org/anim/anim_pcr.html - 30k - Cached

Allele-specific polymerase chain reaction assay

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An allele-specific polymerase chain reaction assay for the ...

File Format: PDF/Adobe Acrobat - View as HTML [Singh O P, Goswami G, Nanda N, Raghavendra K, Chandra D and Subbarao S K 2004 An allele-specific polymerase chain reaction assay ... www.ias.ac.in/jbiosci/sep2004/275.pdf

Basic Polymerase Chain Reaction Protocol

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A Basic Polymerase Chain Reaction Protocol

File Format: PDF/Adobe Acrobat - View as HTML The polymerase chain reaction (PCR) is the cardinal laboratory ... Tutorial entitled Polymerase Chain Reaction. One common choice available to the ... www.idtdna.com/support/technical/TechnicalBulletinPDF/A_Basic_PCR_Protocol.pdf

Homogeneous Polymerase Chain Reaction

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Homogeneous Polymerase Chain Reaction Nucleobase Quenching Assay ...

Homogeneous Polymerase Chain Reaction. Nucleobase Quenching Assay to Detect the 1-kbp. Deletion in CLN3 That Causes Batten Disease ... jmd.amjpathol.org/cgi/reprint/6/3/260.pdf -

Polymerase chain reaction-based construction of cDNA libraries

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Polymerase chain reaction-based construction of cDNA libraries ...

and to construct a polymerase chain reaction (PCR)-. based cDNA library. Materials and methods. Lungworm larvae were isolated from the feces of an ... www.springerlink.com/index/WUUDH735VYF7CGXA.pdf -

Continuous-Flow Polymerase Chain Reaction

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A Continuous-Flow Polymerase Chain Reaction Microchip With ...

rapid micro-polymerase chain reaction system for hepatitis C virus am- ... tion-polymerase chain reaction with cycle number selection," Anal. Chem., vol. ... ieeexplore.ieee.org/iel5/84/33485/01588924.pdf -

PCR : Polymerase Chain Reaction

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PCR : Polymerase Chain Reaction

File Format: PDF/Adobe Acrobat - View as HTML PCR : Polymerase Chain Reaction. 30 - 40 cycles of 3 steps :. Step 1 : denaturation. 1 minut 94 °C. Step 2 : annealing. 45 seconds 54 °C. Step 3 : extension ... users.ugent.be/~avierstr/pdf/PCR.pdf

Polymerase Chain Reaction

The Polymerase Chain Reaction

By Tabitha M. Powledge

It is hard to exaggerate the impact of the polymerase chain reaction. PCR, the quick, easy

method for generating unlimited copies of any fragment of DNA, is one of those

scientific developments that actually deserves timeworn superlatives like "revolutionary"

and "breakthrough."

The Polymerase Chain Reaction

By Tabitha M. Powledge

It is hard to exaggerate the impact of the polymerase chain reaction. PCR, the quick, easy

method for generating unlimited copies of any fragment of DNA, is one of those

scientific developments that actually deserves timeworn superlatives like "revolutionary"

and "breakthrough."

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the polymerase chain reaction

File Format: PDF/Adobe Acrobat - View as HTML The Polymerase Chain Reaction. By Tabitha M. Powledge. It is hard to exaggerate the impact of the polymerase chain reaction. PCR, the quick, easy ... opa.faseb.org/pdf/The%20Polymerase%20Chain%20Reaction.pdf -

RT-PCR assay-cancer cells

Annals of Oncology 13:422-429, 2002
© 2002 European Society for Medical Oncology

Human mammaglobin RT- PCR assay for detection of occult breast cancer cells in hematopoietic products

A. L. Silva, M. J. Tomé, A. E. Correia and J. L. Passos-Coelho⁺

Centro de Investigação de Patobiologia Molecular (CIPM), Instituto Po rtuguês de Oncologia de Francisco Gentil, Lisboa, Po rtugal

Background

The aim of this study was: (i) to evaluate the sensitivity and specificity of mammaglobin as a reverse transcriptase polymerase chain reaction ( RT - PCR) marker of breast cancer cells; (ii) to determine the incidence of tumor cell contamination of hematopoietic samples from patients with breast cancer.

http://annonc.oxfordjournals.org/cgi/content/abstract/13/3/422