Epitope Mapping Using Synthetic Biotin-Labeled Peptides

Epitope Mapping Using Synthetic Biotin-Labeled Peptides
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4278 [Extract] [Full text]

Epitope Mapping of Hemagglutinating Adhesin

Epitope Mapping of

Epitope Mapping of. Hemagglutinating Adhesin. HA-Ag2 of ..... makes it unsuitable for the competition assay. MAb 31A10 ... iai.asm.org/cgi/reprint/60/7/2791.pdf

Epitope Mapping-Antibodies Protocols

Epitope Mapping-Antibodies Protocols - BiocompareEpitope Mapping By
Competition Assay-SUBSCRIPTION REQUIRED Source: CSH Protocols; 2006;
doi:10.1101/pdb.prot4277; Epitope Mapping Using Synthetic ...
www.biocompare.com/protocols/category/3772/Epitope-Mapping-Antibodies.ht
ml - 28k -

Epitope mapping for the anti-rabbit cholesteryl ester transfer ...

Epitope mapping for the anti-rabbit cholesteryl ester transfer ...

Relationship of the binding competition assay to the solid. phase binding assay for the ..... ever, the epitope mapping study showed no indication of ... www.jlr.org/cgi/reprint/40/11/2013.pdf -

Epitope Mapping by Mutagenesis

Epitope Mapping by Mutagenesis Distinguishes between the Two Tertiary
Structures of the Histidine-Containing Protein HPr
Sadhana Sharma, Fawzy Georges, Louis T. J. Delbaere, Jeremy S. Lee,
Rachel E. Klevit, E. Bruce Waygood
Proceedings of the National Academy of Sciences of the United States of
America, Vol. 88, No. 11 (Jun. 1, 1991), pp. 4877-4881 This article
consists of 5 page(s).
View Article Abstract

http://links.jstor.org/sici?sici=0027-8424(19910601)88%3A11%3C4877%3AEMB
MDB%3E2.0.CO%3B2-P

Epitope Mapping by Competition Assay

Epitope Mapping by Competition Assay

Ed Harlow and David Lane

ABSTRACT
The simplest way to determine whether two monoclonal antibodies bind to distinct sites on a protein antigen is to carry out a competition assay. The assay can be used with antibodies that bind both conformational and linear epitopes, and it is most useful in the analysis of monoclonal antibody specificity because polyclonal sera typically recognize multiple different epitopes. This simple, ELISA-based protocol examines the ability of two hypothetical, newly derived monoclonal antibodies, XH1 and XH2, to compete with the well-characterized ZO-1 anti-YFP1 antibody for binding to the target antigen YFP1; hypothetical antibody PC10 is used as a control. First, 96-well plates are coated with the YFP1 antigen and direct binding assays are run to determine that all of the antibody preparations are active and able to react with the immobilized antigen. Second, the competition analysis itself is carried out. Note that the names of antibodies and antigens should be considered as generic.

Epitope Mapping by Competition Assay

Epitope Mapping by Competition Assay
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4277 [Extract] [Full text]

PCR-Based Analysis of Immunoprecipitated Chromatin

PCR-Based Analysis of Immunoprecipitated Chromatin
Alexandre Wagschal, Katia Delaval, Maëlle Pannetier, Philippe Arnaud, and Robert Feil
CSH Protocols; 2007; doi:10.1101/pdb.prot4768 [Abstract] [Full text]

Chromatin Immunoprecipitation-ChIP

Chromatin Immunoprecipitation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications
Alexandre Wagschal, Katia Delaval, Maëlle Pannetier, Philippe Arnaud, and Robert Feil
CSH Protocols; 2007; doi:10.1101/pdb.prot4767 [Abstract] [Full text]

Immunofluorescence of Drosophila S2 Cells

An Approach for Immunofluorescence of Drosophila S2 Cells
Sarkar Angshuman and Schulz Cordula
CSH Protocols; 2007; doi:10.1101/pdb.prot4760 [Abstract] [Full text]

Immunohistochemistry on Cryosections from Embryonic and Adult Zebrafish Eyes

Immunohistochemistry on Cryosections from Embryonic and Adult Zebrafish Eyes
Rosa A. Uribe and Jeffrey M. Gross
CSH Protocols; 2007; doi:10.1101/pdb.prot4779 [Abstract] [Full text]

In Situ Hybridization and Immunohistochemistry

Baskets for In Situ Hybridization and Immunohistochemistry
Hazel L. Sive, Robert M. Grainger, and Richard M. Harland
CSH Protocols; 2007; doi:10.1101/pdb.prot4777 [Abstract] [Full text]

Preparation of Polyclonal and Monoclonal Antibodies

Polyclonal and Monoclonal Antibodies

Adaped from David Bowtell (bowtell@ariel.ucs.unimelb.edu.au)

http://mycoplasmas.vm.iastate.edu/lab_site/methods/immunol/poly_mono_abs
.html

Handbook of Immunochemical Staining Methods

Handbook of Immunochemical Staining Methods: Antibody (Dako)
Immunoglobulins / Polyclonal Antibodies / Monoclonal Antibodies /
Antibody Affinity / Antibody Cross-Reactivity / Antibody Reaction Rates
/ Antibody Stability / Handling of Antibodies / References
http://www.dakousa.com/ishbantibodies.pdf

Antibodies: Immunohistochemistry Protocol

Immunohistochemistry on Cryosections from Embryonic and Adult Zebrafish Eyes
Rosa A. Uribe and Jeffrey M. Gross
CSH Protocols; 2007; doi:10.1101/pdb.prot4779 [Abstract] [Full text]