to Measure Internalization of GPCR's
contributed by Patricia Tsao
Internalization of receptor is measured as the reduction of receptors on
the plasma membrane surface. Surface receptors are labeled with
fluoresent antibodies which is in turn measured using a flow cytometer.
The antibodies recognize an epitope engineered onto the N-terminus of
the GPCR.
The following protocol is optimized for internalization of FLAG-tagged
beta 2-adrenergic receptor (B2AR) stably transfected into HEK293 cells.
Full Protocol Here
http://www.ucsf.edu/vonzast/flow.html
http://209.85.135.104/custom?q=cache:-o87O5wI2JgJ:www.ucsf.edu/vonzast/f
low.html+Flow+Cytometry+protocol&hl=en&ct=clnk&cd=1&client=google-coop
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