Phospho-specific Antibodies Protocol:
Phospho-Specific Antibody development requires careful selection and design of peptide that will be subsequently used to generate antibodies. We provide complete proteomics analyses of your protein of interest for selection of antigenic peptides at no cost to you. Our peptide selection criterion is based on theoretical modeling of protein for determination of application-specific antigenic determinants. Antibodies raised against such peptides are able to detect antigenic epitopes with high specificity on proteins in their native environment. In natural environment proteins have three-dimensional globular structure, some of the antigenic epitopes may be buried inside the protein and will be completely inaccessible to the fairly large antibody molecule. The large size of the antibody molecule will produce sterric hindrance that will not allow proper recognition of antigenic determinants that are not exposed and buried inside. Due to its large size, antibody molecule may not be unable to penetrate the protein matrix. For antibodies that will be used in applications that require detection of antigens in their native or semi native state (confocal microscopy, immunohistochemistry, flowcytometry, and native immunoprecipitation), the antibodies must be raised against an epitope that naturally lies out side the target protein. By careful selection of antigenic peptides that are highly antigenic and naturally lie exposed on the surface of the protein allow us to make antibodies that are function specific.
Phospho-Specific Antibody development requires careful selection and design of peptide that will be subsequently used to generate antibodies. We provide complete proteomics analyses of your protein of interest for selection of antigenic peptides at no cost to you. Our peptide selection criterion is based on theoretical modeling of protein for determination of application-specific antigenic determinants. Antibodies raised against such peptides are able to detect antigenic epitopes with high specificity on proteins in their native environment. In natural environment proteins have three-dimensional globular structure, some of the antigenic epitopes may be buried inside the protein and will be completely inaccessible to the fairly large antibody molecule. The large size of the antibody molecule will produce sterric hindrance that will not allow proper recognition of antigenic determinants that are not exposed and buried inside. Due to its large size, antibody molecule may not be unable to penetrate the protein matrix. For antibodies that will be used in applications that require detection of antigens in their native or semi native state (confocal microscopy, immunohistochemistry, flowcytometry, and native immunoprecipitation), the antibodies must be raised against an epitope that naturally lies out side the target protein. By careful selection of antigenic peptides that are highly antigenic and naturally lie exposed on the surface of the protein allow us to make antibodies that are function specific.
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