PCR IN SITU

PCR IN SITU: NEW TECHNOLOGIES WITH SINGLE CELL RESOLUTION FOR THE DETECTION AND INVESTIGATION OF VIRAL LATENCY AND PERSISTENCE

Katherine A. Staskus, Janet E. Embretson, Ernest F. Retzel,

Janet Beneke and Ashley T. Haase

Copyright � 1994 The Regents of the University of Minnesota

Table of Contents

I. INTRODUCTION AND BACKGROUND
II. THE LENTIVIRUSES AS AN EXPERIMENTAL PROTOTYPE
III. METHODOLOGY
A. Preparation of Cells and Tissues
1. The Balance of Fixation and Pretreatment
B. Primer design
C. Conditions of Amplification
1. Cells in Suspension
2. Cells Within Tissue Sections
D. Detection of Amplified Sequences
IV. APPLICATION AND RELEVANCE TO HUMAN VIRAL DIAGNOSIS AND RESEARCH OF VIRAL PATHOGENESIS
ACKNOWLEDGMENTS
REFERENCES