RT IN SITU PCR

THE FOUNDATION OF SUCCESSFUL RT IN SITU PCR

Gerard J. Nuovo

Director, MGN Medical Research Laboratories, Setauket, New York 11733, USA

Received: 07/25/96; Accepted: 10/02/96; On-line 11/01/96

TABLE OF CONTENTS

1. Abstract

2. Introductory statement

3. The key preparatory steps

3.1. Fixative

3.1.1. Protease digestion

3.1.2. Definition of optimal protease digestion

3.1.3. Definitions of suboptimal and over-digestion

3.1.4. DNase digestion

3.1.5. Direct incorporation of the reporter nucleotide

4. A one step protocol for RT in situ PCR

5. Applications of RT in situ PCR Matrix metalloprotease expression in cancer as a model for RT in situ PCR

5.1. MMP and TIMP expression in cervical cancer

6. Concluding remarks

7. References

8. Entire manuscript

Key words: In situ, PCR, reverse transcriptase

http://www.bioscience.org/1996/v1/c/nuovo1/htmls/list.htm