Detection of Alu by PCR

A Human DNA Fingerprinting Lab Protocol

1994 Cold Spring Harbor Laboratory
DNA Learning Center

In this experiment, polymerase chain reaction (PCR) is used to amplify a nucleotide sequence from chromosome 8 to look for an insertion of a short DNA sequence called Alu within the tissue plasminogen activator (TPA) gene. Although the DNA from different individuals is more alike than different, there are many regions of the human chromosomes t..

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