Introduction of Multiple Mutations Using Overlap Extension PCR

Simultaneous Introduction of Multiple Mutations Using Overlap Extension PCR

BioTechniques 22:28-30 (January 1997)

Introduction of multiple mutations can be accomplished through phage
M13-based site-directed mutagenesis using several oligonucleotides (6). The
major drawback of this method is the low efficiency of generating all the in-
tended mutations simultaneously (7,9). The alternative methods for introducing
multisite mutations are based on poly-merase chain reaction (PCR) (1)

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