PCR
Polymerase Chain Reaction
1) Add the following to a microfuge tube:
10 ul reaction buffer
1 ul 15 uM forward primer
1 ul 15 uM reverse primer
1 ul template DNA
5 ul 2 mM dNTP
8 ul 25 mM MgCl2 or MgSO4 (volume variable)
water (to make up......
Full Details Here
http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/PCR.html
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