Growing up of BAC clones
1. BACs are usually supplied as stabs in agar (2.5 ml screw-top tubes filled with LB agar, stabbed with a toothpick which has been put into a bacterial colony). These are then frozen; at -70C they will keep indefinitely, at -20C for a year or more, at 4C for several months and at room temperature (e.g. during shipping) for a week.
2. Make up 10 ml LB broth + 3 ul of chloramphenicol (dissolved in alcohol, 50 mg/ml, so final concentration is 15 ug/ml, stored at 4C) for each stab which will be grown up....
Read Full Protocol Here
http://www.le.ac.uk/biology/phh4/bacclone.htm
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