How to identify an address:
The membrane is divided into 6 fields (diagram provided). Each field contains 384 squares. The 384 squares represent the row and column identification of the BAC. Within each square there are 16 positions where 8 clones are spotted in duplicate (diagram). The pattern of the spotted clones will generate the plate address of the BAC. To identify your clone, please follow the directions below.
The most complicated part about identifying a clone address is that consecutive plates are not spotted into each field. The 384 well plates are spotted onto the membrane with plates 1-6 spotted into fields 1-6 respectively (duplication pattern 1, see diagram). Since there is a total of 6 fields on the membrane, the cycle will continue with the next six consecutive plates (plates 7 through 12) again being spotted into fields 1 through 6 respectively, but in a different duplication pattern (duplication p.....Full Protocols Here
http://www.genome.clemson.edu/groups/bac/protocols/addressnew.html
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