methylSEQr™ Bisulfite Conversion Kit converts non-methylated cytosines (C) in your DNA sample to uracil (U). This kit is the first step in a three step workflow (step two is PCR amplification; step three is sequence analysis) that enables you to determine which cytosines in your sample are methylated by comparing the sequences of treated vs. non-treated DNA.
This kit enables you to:
Reliable Chemistry
The methylSEQr™ Bisulfite Conversion Kit takes advantage of the different sensitivity of cytosine and methylated cytosine to deamination by bisulfite, in which non-methylated cytosine is converted to uracil and methylated cytosine remains unreactive.
Fast Three-Step Protocol
methylSEQr™ Bisulfite Conversion Kit contains everything you need for a quick and easy three-step protocol:
- Denature your DNA sample with methylSEQr™ Denaturation Buffer.
- Convert unmethylated cytosines to uracils using methylSEQr™ Conversion Reagent.
- Purify your treated DNA sample by centrifugation in a methylSEQr™ Column.
There are three main steps in analyzing the methylation of your DNA sample:
- Bisulfite Conversion with methylSEQr™ Bisulfite Conversion Kit
Convert all non-methylated cytosines in your DNA sample to uracils. - PCR Amplification
Design primers for PCR using Methyl Primer Express® Software
Amplify both the treated and non-treated (control) DNA. During amplification, the uracils in your treated samples convert to thymines (T). To find thermal cyclers, PCR enzymes and kits, and products for PCR reaction purification, go to the PCR section of our catalog. - Sequence Analysis
To detect which cytosines in your sample are methylated, you must compare the sequences of your treated vs. non-treated (control) DNA samples. You have many choices for sequence analysis:
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Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
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