Generalities
Components, PCR cycle, vials
Annealing time and temperature
Choosing PCR primers
How to design the primers
Extension time and temperature
Reaction volumes
Do they influence the results?
Number of PCR products
Multiplexing. How ?
Taq polymerase(s)
Comparisons and concentration
Primer amount
How much primer?
dNTP concentration
Interaction with magnesium
PCR buffers
Comparisons and concentration
MgCl2 concentration
Interaction with dNTP and salt (PCR buffer)
Salt (KCl) concentration
Essential optimization factor
Gel electrophoresis
Agarose and polyacrylamide (PAA)
Designing PCR programs
Customize them for your purpose
Adjuvants in PCR
DMSO, glycerol and BSA
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