Bisulfite-PCR For Restriction Analysis And/or Sequencing

Bisulfite-PCR For Restriction Analysis And/or Sequencing

Source:
Jean-Pierre Issa, The University of Texas M. D. Anderson Cancer Center, Houstan

Bisulfite-PCR followed by restriction is a rapid and semi-quantitative method of analyzing DNA methylation. The PCR products are also suitable for either direct sequencing or cloning and sequencing. The most important step here is primer selection.

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