Primer and Probe Design

Primer and Probe Design
Primer Express software uses a set of default parameters to automatically select primer and probe sets. A summary of the primer and probe design guidelines is shown in Table 1. Even though no probe is required for SYBR Green I dye detection, it is still a good idea to use Primer Express software to select a primer and probe set when designing a SYBR Green I assay. Although no probe will be used, the primers will meet all the.....

Full Details Here
http://dna-9.int-med.uiowa.edu/realtime.htm#design