Real-Time PCR Quantitation

Real-Time PCR Quantitation The ability to monitor the real-time progress of the PCR completely revolutionizes the way one approaches PCR-based quantification of DNA and RNA. Reactions are characterized by the point in time during cycling when amplification of a PCR product is first detected rather than the amount of PCR product accumulated after a fixed number of cycles. The higher the starting copy number of the nucleic acid targ.....

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http://dna-9.int-med.uiowa.edu/realtime.htm#rt%20quant