Real time quantitative PCR

Real time quantitative PCR.

C A Heid, J Stevens, K J Livak, and P M Williams

BioAnalytical Technology Department, Genentech, Inc., South San Francisco, California 94080, USA.

Abstract

We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post- PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays. The real-time PCR method has a very large dynamic range of starting target molecule determination (at least five orders of magnitude). Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.

Full Article Here
http://www.genome.org/cgi/content/abstract/6/10/986