REAL-TIME PCR

REAL-TIME PCR

M. Tevfik Dorak, MD, PhD
 
Dorak MT (Ed): Real-Time PCR (Advanced Methods Series). Oxford: Taylor & Francis, 2006
Real-time reverse-transcriptase (RT) PCR quantitates the initial amount of the template most specifically, sensitively and reproducibly, and is a preferable alternative to other forms of quantitative RT-PCR that detect the amount of final amplified product at the end-point 1 2 (Freeman, 1999; Raeymaekers, 2000). Real-time PCR monitors the fluorescence emitted during the reaction as an indicator of amplicon production during each PCR cycle (ie, in real time) as opposed to the endpoint detection 3,4 (Higuchi, 1992; Higuchi, 1993).

Full Protocol Here
http://dorakmt.tripod.com/genetics/realtime.html