Absolute quantification of mRNA using real-time reverse
transcription polymerase chain reaction assays
S A Bustin
Academic Department of Surgery, St Bartholomew's and the Royal London School of Medicine and
Dentistry, Queen Mary and Westfield College, London E1 1BB, UK; Email: s.a.bustin@mds.qmw.ac.uk
ABSTRACT
The reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive method for the detection of low-abundance mRNA, often obtained
from limited tissue samples. However, it is a complex....
Full Protocol Here
http://www.gene-quantification.de/bustin-2000.pdf
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